Answer:
C.The added epitope disrupts the function of the tagged protein
Explanation:
When an additional sequence is tagged to a protein to use comercial antibodies, there are several reasons why this procedure wouldn't work as expected (note that we're assuming the protein is being expressed but it's not possible to detect it).
For example, the sequence of nucleotide added to codify for the tagged epitope are removed during the RNA processing. In that case, the protein would be expressed without the epitope, so it would be impossible to localize it with the antibodies.
Also, it could be that the new epitope is affecting some way the protein folding, making it not functional. This way, it would be degraded by the cell so it wouldn't be detected.
Another possibility is that the epitope doesn't affects the protein folding nor its function, but during the folding ends up in a conformation that makes it inaccesible for the antibody.
In summary, the way as the possible answers to this question are shown, the correct option seems to be C:<em> The added epitope disrupts the function of the target protein.</em>
Answer:
H2O and O2
Explanation:
water and oxygen is required for photosynthesis
Amoeba acquires it food by endocytosis, that is, a small region in the plasma membrane which ultimately forms a intra-cellular membrane-bound vescicle. It engulfs it food using its pseudopodia (temporary protrusion of the surface of the amoeboid cell used for feeding and movement) and forms a vacuole around it. When the food is already trapped inside, it releases a digestive enzyme to digest it. Intake of liquid particles is pinocytosis (cell drinking) while intake of solid particles is phagocytosis (cell eating).
