Answer:
A serial dilution and the following plating experiment is done in order to determine the actual amount of bacteria/microbes in a specific volume of soil sample. As a standard test, a set volume of the lower dilution is obtained and positioned on a median plate and permitted to increase for the needed amount of time. The amount of colonies is evaluated and thus the overall Colony Forming Units (CFU) is determined by unit volume of the sample plated and therefore the volume of soil sample employed.
Therefore,
The calculation is done using:
CFU/ml = Number of colonies appeared × dilution factor / volume plated
Given,
Number of colonies appeared = 97
dilution factor = 10^(-6)
volume plated = 1/10 = 0.1
This will help us calculate it as:
CFU/ml = 97 * 10^(-6) / 0.1
= 97 * 10^(-7) CFU/ml
This CFU/ml helps determine the amount of bacterial colonies per unit volume of sample plated.
Given,
The original sample as 1g or 1000 mg of soil in its total volume.
Therefore,
The number of bacteria can be calculated using:
Amount of bacteria in original sample
= 97 * 10^(-7) CFU/ml × 1/ 1000 mg
= 9.7 * 10^(-3) CFU/mg
If a flood occurs in the natural rubber environment, it will ruin the rubber production process. The flood would damage a huge percentage of rubber trees if there was a large quantity of restriction. This natural rubber is required for the production of synthetic polymers. Without the rubber, there would be a restricted supply and a significant decline in synthetic polymers due to ecological damage caused by the flood.
Answer: They are also known as <u>B. tranquilizers</u>
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Hope this helps!
