Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
Answer:
Homologous structures provide evidence for common ancestry
Answer:
India was a separate continent until 45 million years ago
Explanation:
India was part of the Gondwanaland continent which was one of the two remnants of Pangaea, while Asia was part of Laurasia, the other remnant of Pangaea. Because these two land masses were separate, the animals and plants were evolving separately for millions of years. India further separated from Gondwanaland, and it was actually a very large island for millions of years, resulting in isolation and development of unique animals and plants because of it. As India was moving it reached Asia and collided with it, thus becoming one land mass. Some Asian species managed to get in India, but mostly in its northern parts, while the central and southern parts remained dominated by the indigenous species. The Himalayan Mountain Range that formed because of the collision also helped in preserving lot of the indigenous species as it became a natural barrier that most species can not go through.
Answer:
A properly designed buffer of trees and shrubs can reduce noise by about five to ten decibels — or about 50 percent as perceived by the human ear, according to the USDA National Agroforestry Center
Explanation: