Answer:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder
Load 12 ul on the gel.
Explanation:
DNA Ladder concentration = 1000 ug/ml
1000 ug DNA in 1 ml DNA Ladder solution → 150 ng DNA = 0.15 ug DNA in..... 0.00015 ml = 0.15 ul DNA Ladder solution
6x DNA Loading Buffer → it has to be diluted by an equal volume 6 times (1 ul LB + 1 ul distilled H2O)
An appropriate volume to load on an average agarose gel is 12 ul, so:
2 ul Loading Buffer + 9.85 ul dH2O + 0.15 ul DNA Ladder = 12 ul
But since 0.15 ul is a very small volume and mistakes could be made while measuring it, let's make double:
4 ul Loading Buffer + 19.70 ul dH2O + 0.30 ul DNA Ladder = 24 ul
And load half of that solution (12 ul) on the gel.
Ecosystems can be large and are made of biomes. Some examples of ecosystems are deserts and forests.
Answer:
The correct answer is eukaryotes engulfed photosynthesizing prokaryotes
Explanation:
The endosymbiotic theory or symbiogenesis is an evolutionary theory that traces the origin of eukaryotic cells from prokaryotic organisms. It explains that some of the organelles (Mitochondria and Chloroplasts) in eukaryotic cells evolved from free-living prokaryotic microbes which were ingested or engulfed. The ingested prokaryotic cells survived within the organism and developed a symbiotic relationship.
According to the theory, chloroplasts was formed when eukaryotes engulfed photosynthesizing prokaryotes and mitochondria was formed when bacteria capable of aerobic respiration were engulfed.
Answer:
It is known as <u>Progressive Muscle Relaxation</u>
<em>b) She alternates muscle tension and relaxation of various muscles in her body.</em>
Explanation:
PMR is a healthy coping skill normally learned in any kind of psychiatric business. For example, I learned PMR while I was inpatient at a behavioral health unit. The way we did PMR was that we'd sit up in our chairs and start from the toes and on up. We squeezed our toes together for a few seconds and then released and then we'd do it a second time. After the toes, it would move up to the feet and we'd use the same process for that and for the rest of the joints.
You can reduce the amount of pollutants in your home by improving air circulation.
