The correct answers are: the insertion of a DNA sequence into the targeted gene and deletions of the targeted gene
Cas9 is an enzyme endonuclease (which means it cuts nucleotides) that is part of the CRISPR-Cas9 complex- genome editing tool that can remove, add or alter sections of the DNA sequence.
Cas9 recognize the sequence that is going to be cut thanks to guide RNA- pre-designed RNA sequence complementary to sequence of interest. DNA repair machinery will try to fix the “damage” but it can add (insert) or delete some nucleotides.
Transpiration<span> is the process which moisture is carried through plants from roots to small pores on the under part of leaves, where it changes to water vapor and is released to the earth. </span>Transpiration<span> is essentially evaporation of </span>water<span> from plant leaves.</span>
Answer:
What is the effect of power/magnification on the frequency and size of organelles under a microscope?
Explanation:
Organelles within the cell are responsible for carrying out various functions. Some cells are more specialized than others, and may have particular organelles at a higher frequency, or showing a variation in size; sub-cellular structures become more visible at higher magnifications under the microscope.
Hypotheses:
- organelle A's frequency decreases while B's frequency increases at higher magnifications
- organelle A's size increases while B's size decreases at higher magnifications
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<em>Dependent variables: size and frequency cell organelles</em>
<em>Independent variable: power/magnification at low (x4), medium(x10) and high (x40)</em>
<em>Controlled variables: Type of organelles, microscope used, cell examined, </em>
Method:
1. Examine the organelles A and B in a cell mounted on a slide; use the fine adjustment to focus on the cell.
- Frequency: What is the average number of organelle A versus B, seen at low (x4), medium (x10) and high (x40) magnifications?
- Size: Measure the average diameter of organelle A versus B using an ocular micrometer at low, medium and high magnification.
2. Record and tabulate observations.
I BELIEVE the answer is D) 4.
Answer:
<h2>
Interphase : divided into three phases, i) G1 phase, ii) S phase and iii) G2 phase.</h2><h2>Mitotic phase: i) prophase, ii) metaphase, iii) anaphase and v) telophase.</h2>
Explanation:
interphase : divided into three phases, i) G1 phase, ii) S phase and iii) G2 phase.
G1 phase: cell decide whether to divide or not and prepare itself for replication of DNA and arrange replication machinery. otherwise it goes to G 0 phase.
S phase: DNA replication occurs in this phase.
G2 phase: cell duplicates all their contents and prepares for mitotic phase.
Mitotic phase:
i) prophase- chromosome condensation occurs,
ii) metaphase - chromosome arranges in meta-plate and spindle binds to each chromosomes at centromere.
iii) anaphase- chromosome separates from sister chromatids.
iv) telophase- chromosome moves to each ends and formation of nuclear membrane begins.
cytokinesis: there is division of cytoplasm and forming two daughter cells.
