Cas9 cleaves both strands of the target DNA. Which of the following could occur when cells will try to repair the damage after c
leavage by Cas9? (Select all that apply.)mutations that result in changes to the open reading frame (ORF) of the targeted geneloss-of-function mutations within the targeted genethe insertion of a DNA sequence into the targeted geneno change in the targeted genedeletions of the targeted gene
The correct answers are: the insertion of a DNA sequence into the targeted gene and deletions of the targeted gene
Cas9 is an enzyme endonuclease (which means it cuts nucleotides) that is part of the CRISPR-Cas9 complex- genome editing tool that can remove, add or alter sections of the DNA sequence.
Cas9 recognize the sequence that is going to be cut thanks to guide RNA- pre-designed RNA sequence complementary to sequence of interest. DNA repair machinery will try to fix the “damage” but it can add (insert) or delete some nucleotides.
