Question

Typically, urea broth is filter-sterilized because autoclaving breaks down the urea. However, even unsterilized broth rarely produces false-positive results. Why do you think this is true?

Answer 1

Answer:

When organisms use urea, ammonia is generated during incubation, which makes the reaction of these media alkaline

Explanation:

Urea broth preparation:

Suspend 3.87 grams of the medium in 100 ml of distilled water without heating. When the powder dissolves, sterilize by filtration. Dispense quantities of 0.5 to 2 ml in small sterile tubes. Larger volumes may be used but the reactions will be slower. Do not sterilize in autoclave.

Do not boil the medium.

If no filter is available, the medium can be sterilized at 100-110 ° C for 10 minutes. If the medium is prepared and inoculated immediately, it provides good results without sterilization.