Answer:
Pioneer species is the general name for the first organisms to move into a disturbed environment or to grow after a volcanic eruption covers an area with lava.
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Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
1. Energy
2.tri-phosphate
3. bond
i take AP bio so make this a brainliest answer
D : the shape of a solid does not change when it is put into a container
Answer:
- Autotrophs are usually defined as those that can prepare their own food by carrying out the process of photosynthesis, but heterotrophs cannot prepare their own food and are directly dependent on the autotrophs for food. Examples of autotrophs are plants and trees, and examples of heterotrophs are animals and human beings.
- Autotrophs are the lowest organisms in the trophic level, where they produce the food for the consumers (heterotrophs). On the other hand, the heterotrophs lie above the autotrophs and when they consume their food, they obtain only 10% of the energy, and the remaining energy is released into the environment.
- Autotrophs can make organic substances by the use of inorganic molecules, but heterotrophs cannot make these substances. They are only dependent on the molecules prepared by these autotrophs.
Thus, these are three of the facts regarding autotrophs and heterotrophs existing on earth that are true.