Answer: A double restriction digest
Explanation: A double restriction digest is a process were two restriction enzymes are used to digest DNA in a single reaction.in double restriction digest you will observe two bands,while in single restriction digest you will observe just one band. In double restriction digest no
Self - ligating plasmid because it create mismatch ends. It also aids in directional in section while single disgestion you have to screen your clones to be sure to get your plasmid with correct insert orientation.
The correct answer for your question is C.
Answer:
Plague is caused by Yersinia pestis. It is unicellular and placed in the bacteria domain.
Explanation:
Plague is caused by bacteria Yersinia pestis , a zoonotic bacteria usually found in the small mammals and their fleas. It is a gram-negative, nonmotile, rod-shaped, coccobacillus bacteria, with no spores. It is a facultative anaerobic organism that can infect humans via the Oriental rat flea.Y. pestis was discovered in 1894 by Alexandre Yersin, a Swiss/French physician and bacteriologist from the Pasteur Institute, during an epidemic of the plague in Hong Kong. Yersin was a member of the Pasteur school of thought. Kitasato Shibasaburō, a German-trained Japanese bacteriologist who practised Koch's methodology, was also engaged at the time in finding the causative agent of the plague. However, Yersin actually linked plague with Y. pestis. named Pasteurella pestis in the past, the organism was renamed Yersinia pestis in 1944.
<span>Cells primarily recognize exposed hydrophobic patches when identifying misfolded proteins. This is very prevalent in bacteria and I would assume that similar mechanisms are used in eukaryotes. There is also a system where misfolded proteins are tagged by different glycosylation patterns, but I don't remember much about that part.</span>
