Answer:
Explanation:
Bacterial count in stock- 1.85x10^6 cfu/ml
Dilution methods
Take 100 uL or (0.1ml) from stock and add to 900ul (0.9ml) saline and mixed it- this makes 10^1dilution.
Now take 100ul from 10^1 dilution and add to next 900ul saline this is 10^2 dilution, similarly do upto 10^5 dilution.
Then take 100ul from 10^ 4 and 10^5 dilution seperately and plate on LB agar plate seperetely and count the colonies.
Cfu/ml formula= (No.of colonies x dilution factor)/0.1 ml
So suppose, 18 colonies formed on 10^4 dilution then total no. Of cells in stock will be 18x10^4/ 0.1= 18x10^5 cfu/ml.
If we dilute 10^4 or 10^5 that's leads to colony count of 18-19 colonies on 10^4 dilution while 2 colonies should come on plate of 10^5 dilution.
Answer:
That there used to be an abundance of plants and animals that lived there. Does this help?
Explanation:
Coal forms from dead things from the past being subjected to high pressure.
Are there any options you can provide?
***see attached pic***
Some helpful tips:
The nucleus is always going to be the big, spherical shape.
The ER is always going to be a weird, folded looking structure attached to the nucleus (rough = has ribosomes on it which are usually represented by little dots, smooth = has no ribosomes on it aka no dots)
The GA looks like the ER but it is not going to be attached to the nucleus like the ER is.
The mitochondria is always going to be pill-shaped and have weird folds inside of it.
<span>lactose Hope i helped :)</span>