Answer:
The correct answer is: d. REs activity is best preserved by freezing the RE and thawing it out for brief periods of time as needed.
Explanation:
- Restriction enzymes can be defined as endonucleases that can recognize specific nucleotide sequence in a DNA (Deoxyribonucleic acid) sequence and cause breakage of phosphodiester bond in the DNA at the target site. Hence, it has the capacity of cleaving a DNA sequence.
- These are made up of proteins which are required to be present in the accurately folded conformation in order to function.
- Any form of alteration in the three-dimensional structure of the protein induced due to physical, chemical, mechanical or physiological factors can cause denaturation of the protein which in turn can render the protein non-functional.
- Freezing of restriction enzymes (REs) directly without the use of glycerol will cause the movement of water molecules within the proteins as well layering of water molecules on the protein surface.
- At low temperature these water molecules change to ice crystals which can alter the conformation of the protein or can cause breakage of peptide bonds in the proteins by physical force.
- This can cause the protein to get denatured and hence non-functional.
- Hence, it is never advisable to preserve REs by freezing directly without glycerol.
- Glycerol forms a layer around the protein molecules and prevents the protein molecules from coming into direct contact with the water molecules and hence prevents protein denaturation.
I am confident that the answer should be.....<span>A protein acts as a pump and moves calcium ions from an area of low concentration to an area of high concentration.
Hope this helps.
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Answer:
about nine mouths hope this helps
Explanation:
I think it is beneficial for the brain to be connected to the contralateral side of the body because of the position and connection to the brain that our eyes have. Because light from the right visual fields strikes the left side of the eye and light from the left side strikes the right side of the eye, the connections allow visual information from both sides of the body to reach the brain.
Answer:
SARS-CoV-2 is the seventh coronavirus strain known to infect humans and it causes respiratory disease in humans. These viruses infect humans and are currently causing a pandemic in the world.
A pathogen is an infectious organism that causes illness or disease to its host. There are many types of pathogens that infect the body of the host that disrupts the normal physiological process of an organism. SARS CoV2 is also a pathogen as it infects the human body and disrupts its physiology and causes disease.
