Confirm that the sample has both helicase and primase activities, but not the ability to synthesize DNA
- The replisome is a multiprotein-RNA complex that is required for the replication of DNA. The replisome consists of different proteins which include:
1. Helicase enzymes that unwind and separate the DNA strand
2. Replication factor C (RFC), a DNA-dependent ATP that acts as an activator of DNA polymerase.
3. Proliferating cell nuclear antigen (PCNA)
4. DNA gyrase/topoisomerase that cuts and reseals DNA strands, which is essential for DNA synthesis.
5. Primase enzyme that synthesizes short RNA fragments called primers.
6. DNA polymerase III, an enzyme that catalyzes the polymerization of deoxyribonucleotides into a DNA strand.
7. DNA ligases enzymes that form phosphodiester bonds at a single-strand break in DNA
- A primosome is a multiprotein complex responsible for creating RNA primers on single-stranded DNA (ssDNA) during the replication process.
- The primosome is composed of different primase and helicase enzymes. In<em> Escherichia coli</em>, it consists of PriA helicase, PriB, PriC, DnaB helicase, DnaG (encoded by the dnaG gene), DnaC and DnaT primase.
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Let’s assume a wire-hair terrier is a true breed for wire hair with alleles (BB) and a smooth-hair terrier is true breed for smooth hair (bb). Allele B is dominant over its allele b. When these two terrier mated and produce offspring wire-hair terrier and smooth-hair terrier in the ratio 3:1.
Based on this result it can be inferred that –
a. The pattern of inheritance is monohybrid cross.
b.Genotype of wire-hair terrier (1BB and 2 Bb) and genotype of smooth-hair terrier is (bb)
c. Phenotype ratio will be 3:1 and genotypic ratio will be 1:2:1.
For eukaryotes to produce the next generation through sexual reproduction, two cells must contribute genetic material.
Sexual reproduction, the process through which two individuals of different sexes combine their genetic information to create new species. In the majority of organisms, reproductive cells called gametes, which mate to create a diploid zygote, have chromosomes in their nuclei that carry the genetic material. The zygote grows into a new person. The most common method of reproduction among living things is sexual reproduction. A remarkable variety of offspring, each with a genetic make-up different from that of its parents, may result from sexual reproduction because it allows the rearranging of genetic material both within and between members of one generation. Sexual reproduction is involved.
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Answer:
0 black rabbits, 0 white rabbits, 8 grey rabbits
Explanation:
A true-breeding black rabbit is crossed with a true-breeding white rabbit to produce an F1 generation of 16 individuals. If the black color trait is dominant, 0 black rabbits, 0 white rabbits, 8 grey rabbits outcomes represents the expected phenotype of an F1 generation cross.