You need to search your pKa values for Asn (2.14, 8.75), Gly (2.35, 9.78) and Leu(2.33, 9.74), the first value corresponding to -COOH, the second to -NH3 (a third value would correspond to an R group, but in this case that does not apply), and we'll build a table to find the charges for your possible dissociated groups at indicated pH (7), we need to remember that having a pKa lower than the pH will give us a negative charge, having a pKa bigger than pH will give us a positive charge:
Now that we have our table we'll sketch our peptide's structure:
<em>HN-Asn-Gly-Leu-COOH</em>
This will allow us to see what groups will be free to react to the pH's value, and which groups are not reacting to pH because are forming the bond between amino acids. In this particular example only -NH group in Ans and -COOH in Leu are exposed to pH, we'll look for these charges in the table and add them to find the net charge:
+1 (HN-Asn)
-1 (Leu-COOH)
=0
The net charge is 0!
I hope you find this information useful and interesting! Good luck!
