Substitution mutations are the point mutations that are the mutations that least likely affect the trait of an organism or have no effect at all. Substitution mutations take place when there is only one nucleotide base is substituted by another nucleotide base in the DNA or RNA of an organism's genome.
In this case, there is no effect found on the protein formed by these DNA sequences. These substitution mutations generally found in somatic cells and have little to no effect at all.