Confirm that the sample has both helicase and primase activities, but not the ability to synthesize DNA
- The replisome is a multiprotein-RNA complex that is required for the replication of DNA. The replisome consists of different proteins which include:
1. Helicase enzymes that unwind and separate the DNA strand
2. Replication factor C (RFC), a DNA-dependent ATP that acts as an activator of DNA polymerase.
3. Proliferating cell nuclear antigen (PCNA)
4. DNA gyrase/topoisomerase that cuts and reseals DNA strands, which is essential for DNA synthesis.
5. Primase enzyme that synthesizes short RNA fragments called primers.
6. DNA polymerase III, an enzyme that catalyzes the polymerization of deoxyribonucleotides into a DNA strand.
7. DNA ligases enzymes that form phosphodiester bonds at a single-strand break in DNA
- A primosome is a multiprotein complex responsible for creating RNA primers on single-stranded DNA (ssDNA) during the replication process.
- The primosome is composed of different primase and helicase enzymes. In<em> Escherichia coli</em>, it consists of PriA helicase, PriB, PriC, DnaB helicase, DnaG (encoded by the dnaG gene), DnaC and DnaT primase.
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Firstly, the enzyme, DNA Helicase breaks the Hydrogen bonds between a specific region of the DNA molecule and unwinds the duplex DNA molecule at the targeted gene sequence site.
Then, RNA Polymerase binds at the 3` site of the gene sequence of the sense/coding strand(only one strand) that acts as the template for mRNA synthesis. RNA Polymerase then matches free nucleotides by complementary base-pairing(A-U & C=G), working in the 5`to3` direction. Hydrogen bonds then form between the complementary bases, holding the nucleotides in place. Each free adjacent nucleotide is joined at 3` ends by Condensation reactions with the other to form Phosphodiester bonds.
Once the mRNA molecule is formed, it is separated from the synthesis complex and leaves the nucleus through pores in the nuclear envelope to moves towards ribosomes for Translation.
Lastly, the DNA double helix rewinds by forming Hydrogen Bonds.
The answer is “B” because the blowing through the straw would be like the wind
Answer:
DNA and protein
Explanation:
two biomolecules are most often
analyzed to establish homologies between
different species are DNA and proteins