Complete question:
The genes for miniature wings (m) and garnet eyes (g) are approximately 8 map units apart on chromosome 1 in Drosophila. Phenotypically wild-type females (m + g / mg +) were mated to miniature-winged males with garnet eyes.
<u>Part A</u>
Which of the following phenotypic classes reflect offspring that were generated as a result of a crossover event?
Select all that apply.
-
garnet eyes
- wild type
- miniature wings
- miniature wings, garnet eyes
<u>Part B</u>
If 800 offspring were produced from the cross, in what numbers would you expect the following phenotypes?
__wild type : __ miniature wings : __ garnet eyes : __ miniature wings, garnet eyes
Enter your answer as the number of flies of each phenotype separated by a colon (example: 100:300:100:300).
Answer:
<u>Part A</u>:
2. wild type (m+g+/mg)
4. miniature wings, garnet eyes (mg/mg)
<u>Part B:</u>
32:368:368:32
Explanation:
When calculating the recombination frequency, we need to know that 1% of recombinations = 1 map unit = 1cm. And that the maximum recombination frequency is always 50%.
The map unit is the distance between the pair of genes for which every 100 meiotic products, only one results in a recombinant one.
So, en the exposed example:
<u>PART A</u>:
First, we need to Identify the gametes for the individual with the genotype m + g / mg + and label parental and recombinants
Gametes:
- m+ g parental type
- m g+ parental type
- m+ g+ recombinant type
- m g recombinant type
Knowing that the only possible gamete type for the other parental is mg, then we can identify the phenotypic classes of the offspring that were generated as a result of a crossover event. These would be
2. wild type (m+g+/mg)
4. miniature wings, garnet eyes (mg/mg)
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<u>PART B:</u>
- We know that the total number of individuals equals 800.
- We also know that the distance between genes equals 8 MU.
To calculate the number of the phenotypes among the offsprings,
8 map units = 8% of recombination in total
= 8/2% m+g+/mg + 8/2% mg/mg
= 4% + 4%
The recombination frequency of m+g+/mg equals 0.04
The recombination frequency of mg/mg equals 0.04.
100% - 8% = 92% of parental in total =
= 92/2% of m+ g/mg + 92/2% m g+/mg
= 46% + 46%
The recombination frequency of m+g/mg equals 0.46
The recombination frequency of mg+/mg equals 0.46.
The frequency for each ggenotype is:
- F (m+g+/mg) = 0.04
- F (mg/mg) = 0.04.
- F (m+g/mg) = 0.46
- F (mg+/mg) = 0.46.
The number of individuals with each genotype is:
- m+g+/mg = 0.04 x 800 = 32
- mg/mg = 0.04 x 800 = 32
- m+g/mg = 0.46 x 800 = 368
- m g+/mg = 0.46 x 800 = 368
32 wild type : 368 miniature wings : 368 garnet eyes : 32 miniature wings, garnet eyes