Collagen supported cells :)
Answer:
14 CO₂ will be released in the second turn of the cycle
Explanation:
<u>Complete question goes like this</u>, "<em>The CO2 produced in one round of the citric acid cycle does not originate in the acetyl carbons that entered that round. If acetyl-CoA is labeled with 14C at the carbonyl carbon, how many rounds of the cycle are required before 14CO2 is released?</em>"
<u>The answer to this is</u>;
- The labeled Acetyl of Acetyl-CoA becomes the terminal carbon (C4) of succinyl-CoA (which becomes succinate that is a symmetrical four carbon diprotic dicarboxylic acid from alpha-ketoglutarate).
- Succinate converts into fumarate. Fumarate converts into malate, and malate converts into oxaloacetate. Because succinate is symmetrical, the oxaloacetate can have the label at C1 or C4.
- When these condense with acetyl-CoA to begin the second round of the cycle, both of these carbons are discharged as CO2 during the isocitrate dehydrogenase and alpha-ketoglutarate dehydrogenase reactions (formation of alpha-ketoglutarate and succinyl-CoA respectively).
Hence, 14 CO₂ will be released in the second turn of the cycle.
Answer:
To determine the effect of toxin binding, you perform a Competitive Binding Experiment
Explanation:
Competitive binding experiments are very relevant when matters relating to cellular and or molecular toxicity are concerned.
They are used to determine the binding of a single concentration of labeled ligand in the presence of various concentrations of unlabeled ligand.
They are important because:
1. Validate a direct binding assay
2. Confirm whether or not a drug binds to the receptor
3. determine and explore how low-affinity drugs and receptors interact and it's resultant effects
Cheers
I believe you're looking for "chemical digestion".
Chemical digestions is where food that we ate is digested into simpler and soluble molecules that can be absorbed by our body. Enzymes are used as a catalyst to speed up the reaction.
One example is the digestion of lipids. Enzyme named lipase is used to speed up the digestion of lipids into 3 fatty acid molecules and 1 glycerol. This way, our body can absorb these simple molecules, and use them in order to make our body function.
On the other hand, physical digestion does not include enzymes, such as chewing in our mouth, therefore, don't mix them up!
