Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
It is rare for soft tissue to become a fossil because the soft parts can be eaten by other animals. Hope this helps!
Water enters the soil in a process called infiltration, and then moves downward through the soil in a process called percolation.
Answer: The answer is .007 I believe. If I'm right please mark me brainiest
Explanation:
I just took the test and some teachers are taking .008 but some only take .007 so yea
Answer:
C. Bronchial
Explanation:
The different types of lung sounds, also known as breath sounds differ from each other with respect to their location, pitch, relative duration, and intensity.
The bronchial breath sounds are heard just above the clavicle on either side of sternum bone and are characterized by loud amplitude, harsh quality, and high pitch. The bronchial breath sounds are hollow tubular.
The bronchial breath sounds have a short period of silence between the inspiratory and expiratory sounds. The expiratory bronchial sounds last longer than the inspiratory bronchial sounds (I<E).