If you mean higher boiling point than it is oil.
Step 1: Isolate the two kinds of DNA.
Step 2: Treat the plasmid and foreign DNA with the same restriction enzyme.
Step 3: Mix the foreign DNA with chopped plasmids.
Step 4: Add DNA ligase.
The correct answer is "trans fatty acids".
Trans fatty acids (colloquially as trans fats) are a type of unsaturated fatty acids wherein instead having its double bonds in the cis configuration giving the fatty acid "kinks" in the molecules making it more soluble; the double bonds in trans fats are in the trans configuration (hence the name) and this will not give the molecule kinks making it less soluble. Less soluble trans fats are notorious in depositing in peripheral arteries as well as in coronary arteries that increase the risk for developing atherosclerosis.
In order to "invest" in later stages to get more energy per glucose molecule. It could also be because that is how it is done, it wouldn't be as efficient unless these stages were performed.
hope this helps!