You would add H+ ions if you added an acid. Thus lowering the pH.
Answer:
a) Genes b and c are farthest apart.
Explanation:
Transformation occurs when a competent bacteria cell takes up genetic material from the environment. Usually a donor cell donates its gene fragment which is then incorporated into the chromosome or plasmid of recipient bacterial cell.
Cotransformation occurs when two genes are taken up together by the recipient. The closer the genes lie to each other, more are the chances of them being taken up together. Contransformation frequency will be higher if two genes are close to each other. Here, cotransformation frequencies between three genes are given. Amongst them, the lowest frequency is 0.0064% which is present between gene b and c. Hence, gene b and c are the farthest apart.
A Negative stain such as India ink or Congo red-Look for the presence of a capsule.
This stain method is usually used to stain the area around the microorganism.
B The Gram stain-Divide bacteria into two groups based on cell wall structure (thick vs. thin).
This is type of differential staining used to distinguish organisms based on their staining properties. Gram + and Gram- bacteria stain different because of different cell wall structure.
C The Ziehl-Neelsen Acid-fast stain- Identify bacteria with waxy cell walls such as: Mycobacterium tuberculosis (the cause of TB).
This is also differential staining method which uses heat and phenol to derive dye into the cells with lipid-rich walls.
D Simple stain with a basic dye-Stain microbes a bright color to make it easier to see them in bright field microscopy.
One dye is used in simple staining in order to determinate the size, shape and arrangement of the cells.
E The Schaeffer-Fulton Endospore stain-Identify Bacillus or Clostridium species, such as the causative agents of anthrax, botulism, tetanus and gangrene.
This is a special type of staining only used for the bacteria that can form endospores. Bacteria are first treated with heat and then with malachite green, which is very strong stain that can penetrate endospores.
Lipids form the bilayer of cell membrane which act as a partially permeable mmbrane of cell to control the substances moving in and out of the cell.
