Answer:
Background
During the course of a bacterial infection, the rapid identification of the causative agent(s) is necessary for the determination of effective treatment options. We have developed a method based on a modified broad-range PCR and an oligonucleotide microarray for the simultaneous detection and identification of 12 bacterial pathogens at the species level. The broad-range PCR primer mixture was designed using conserved regions of the bacterial topoisomerase genes gyrB and parE. The primer design allowed the use of a novel DNA amplification method, which produced labeled, single-stranded DNA suitable for microarray hybridization. The probes on the microarray were designed from the alignments of species- or genus-specific variable regions of the gyrB and parE genes flanked by the primers. We included mecA-specific primers and probes in the same assay to indicate the presence of methicillin resistance in the bacterial species. The feasibility of this assay in routine diagnostic testing was evaluated using 146 blood culture positive and 40 blood culture negative samples.
Explanation:
Results
Comparison of our results with those of a conventional culture-based method revealed a sensitivity of 96% (initial sensitivity of 82%) and specificity of 98%. Furthermore, only one cross-reaction was observed upon investigating 102 culture isolates from 70 untargeted bacteria. The total assay time was only three hours, including the time required for the DNA extraction, PCR and microarray steps in sequence.
Explanation:
The act of aspiring or ardently desiring; an ardent wish or desire, chiefly after what is elevated or spiritual (with common adjunct adpositions being to or of)
Answer:
the cell cycle
Explanation:
Image of the cell cycle. Interphase is composed of G1 phase (cell growth), followed by S phase (DNA synthesis), followed by G2 phase (cell growth). At the end of interphase comes the mitotic phase, which is made up of mitosis and cytokinesis and leads to the formation of two daughter cells.
Answer: Subsistence agriculture.
Explanation: Subsistence agriculture is a type of farming in which farmers cultivate and grow food crops and livestocks to meet their needs and that of their family.
In this type of agriculture, farm products is aimed to survival and it doesn't involve use of large-scale irrigation, chemical fertilizer, or machinery and technology.
Explanation:
Your stomach is a sac-like organ with strong muscular walls. In addition to holding food, it serves as the mixer and grinder of food. Your stomach makes acid and powerful enzymes that break the food down and change it to a liquid or paste. From there, food moves to your small intestine
