Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Answer: Liver
Glucose is the most important fuel source for the body, specifically the brain. It is absorbed through the
mucosal lining into the epithelial cells of the intestine by active transport
via sodium-dependent hexose
transporter. From the epithelial cells, glucose is moved into the surrounding capillaries
by facilitated diffusion into the liver. Once in the liver, glucose is stored as glycogen.
During the process of transcription, mRNA decodes information from the original DNA master plan to build proteins in the ribosomes. If it helped please mark as the brainliest answer.
Didn't you say that the water at 39 degrees is more dense than water at its freezing point? I think the reason organisms would die is because the water already starting to structure in a crystal form, when it starts to freeze into ice. This probably why it kills the organisms, is because the water ceases to allow the organism to function at those temperatures.