Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
Answer:
Lithospheric
Explanation:
Lithospheric plates are part of a planetary scale thermal convection system. The energy source for plate tectonics is Earth's internal heat while the forces moving the plates are the “ridge push” and “slab pull” gravity forces
Answer:
Please find the explanation below
Explanation:
Sodium (Na) and Chlorine (Cl) are two electrolytes that play important role in the absorption of water and nutrients in the small intestine. However, as mentioned in the question, the release of sodium and chlorine would lead to a loss of water in the intestines. This is because of the OSMOTIC phenomenon.
Osmosis is the movement of water molecules from a region of high concentration of water/low concentration of solute to a region of low concentration of water/high concentration of solute via a semipermeable membrane. In this case, the Na+ and Cl- ions serve as the solutes, which when released out of the intestine causes the solution in the intestines to be HYPOTONIC compared to the intestinal environment. This causes an OSMOTIC GRADIENT.
This osmotic gradient i.e. difference in concentration provokes osmotic flow of water from the intestines, which has a low solute/high water concentration, to the outside of the intestines, which has a high solute/low water concentration. Hence, water is lost from the intestine because the solute concentration becomes low when sodium and chlorine ions are released.
Answer:
The amount of sunlight
Explanation:
solar energy comes from the sun and uv rays this is my explanation
I believe you are referring to the Disney cartoon "The Aristocats."
At one moment during the film, one of the cats called Marie falls off the truck and into a river. However, she is saved by one of the Aristocats called Thomas O'Malley, a feral cat who falls in love with the Duchess and acts as a fatherly figure to her kittens.
