Pioneer species are found in area's undergoing secondary succession. For example ecosystems being established by: reducing a fire, deforestation; which quickly colonize open spaces supported by vegetation.
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when youre feelinsgs subside and shadows still remain
gons and ruse
Xylem is one of the two types of transport tissue in vascular plants, phloem being the other. The basic function of xylem is to transport water from roots to stems and leaves, but it also transports nutrients.
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geologists use rock samples and direct evidence to prove what earth's structure is like.
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Bacterial count in stock- 1.85x10^6 cfu/ml
Dilution methods
Take 100 uL or (0.1ml) from stock and add to 900ul (0.9ml) saline and mixed it- this makes 10^1dilution.
Now take 100ul from 10^1 dilution and add to next 900ul saline this is 10^2 dilution, similarly do upto 10^5 dilution.
Then take 100ul from 10^ 4 and 10^5 dilution seperately and plate on LB agar plate seperetely and count the colonies.
Cfu/ml formula= (No.of colonies x dilution factor)/0.1 ml
So suppose, 18 colonies formed on 10^4 dilution then total no. Of cells in stock will be 18x10^4/ 0.1= 18x10^5 cfu/ml.
If we dilute 10^4 or 10^5 that's leads to colony count of 18-19 colonies on 10^4 dilution while 2 colonies should come on plate of 10^5 dilution.