Answer:
SEM ANSWER
Explanation:
Electron microscopy is a powerful tool in the field of microbiology. It has played a key role in the rapid diagnosis of viruses in patient samples and has contributed significantly to the clarification of virus structure and function, helping to guide the public health response to emerging viral infections. In the present study, we used scanning electron microscopy (SEM) to study the infectious cycle of SARS-CoV-2 in Vero E6 cells and we controlled some key findings by classical transmission electronic microscopy (TEM). The replication cycle of the virus was followed from 1 to 36 h post-infection. Our results revealed that SARS-CoV-2 infected the cells through membrane fusion. Particles are formed in the peri-nuclear region from a budding of the endoplasmic reticulum-Golgi apparatus complex into morphogenesis matrix vesicae. New SARS-CoV-2 particles were expelled from the cells, through cell lysis or by fusion of virus containing vacuoles with the cell plasma membrane. Overall, this cycle is highly comparable to that of SARS-CoV. By providing a detailed and complete SARS-CoV-2 infectious cycle, SEM proves to be a very rapid and efficient tool compared to classical TEM.
D. skeletal muscle and cardiac muscle
Answer:
A,B and E
Explanation:
- I+: I refers to the lac repressor, the + indicates that the repressor is functional
- P+: P refers to the promoter region and the + indicates that the promoter is functional
- Oc: O refers to the operator region, but the Oc indicates that the operator is constitutive ie. that it is always on/ can't be turned off.
- Z-: Z refers to the LacZ gene which produces β-galactosidase. Z- indicates that this gene is non-functional
- Y+: Y refers to the LacY gene which produces the permease protein. Y+ indicates that this gene is functional
So based on the breakdown above:
- C is not true because the lac operon is an inducible system. The operator needs to be able to be repressed. In this case the operator is constitutive (always on) which is not the correct form.
- D is not true because Z-, no β-galactosidase is produced.
Only A, B and E apply.
