Answer:
The first attached figure below shows the design of an agarose gel with four sequencing reactions. The second figure presents a photo of an agarose gel, so that you can better understand how the bands are represented in this gel.
Explanation:
To view the bands of four sequencing reactions on an agarose gel, you will need to use a melted agarose gel, plastic combs suitable for that reaction and a container suitable for that type of gel. You will place the plastic combs in the container and pour all the gel into the vat and wait for the melted gel to solidify. The plastic combs will form holes in the hardened gel where the DNA samples will be placed.
Once the gel is hardened, you will remove the plastic combs and begin to apply the sequenced DNA.
The sequenced DNA samples will be mixed with a dye, usually bromophenol blue, which will allow you to visualize the bands formed on the gel. You will also apply the dye to a sample without DNA containing only the dye, which serves as a comparison for the size of the bands.
Each sample of DNA will be plated in the column of holes formed by the plastic combs. Then, this container, with the gel, will be placed in a larger container that contains a loading buffer. The larger container will be closed and an electric field will be applied that will force the DNA samples to be moved from one pole to another inside the container, in this case, the samples leave the negative pole for the positive pole.
After a few minutes, it is possible to visualize the DNA displacement and at the end of the procedure it will be possible to visualize the formation of bands as shown in the drawing and in the figure below. The size of these bands can be compared and analyzed.
Answer:
a prezygotic barrier
Explanation:
Both wild tigers and lions are said to be reproductively isolated.
Reproductive isolation is defined as the existence of barrier between organisms of different species such that they cannot reproduce.
The mechanisms of reproductive isolation varies. While some act to prevent the formation of zygotes (in case of mating) between organisms of different species, some ensures that the resulting zygotes are invalid.
Reproductive isolation mechanisms that act to prevent fertilization and formation of zygotes are referred to as prezygotic mechanisms while those that ensure that invalid zygotes result (in case of successful fertilization) are referred to post zygotic mechanisms.
<em>The natural differences in the ranges of wild tigers and lions ensure that both animals do not mate with each other, let alone results in zygote. This is a form of pre-zygotic reproductive isolation mechanism.</em>
Answer:
Apparently 0 is the answer which makes sense
Explanation:
https://www.tiger-algebra.com/drill/(x-5)(x_1)/3(x_1)=x-5/3/
The correct answer is "identifies the feelings underlying the acting-out behaviour ".
This statement shows that the adolescent is recognizing the feelings and is ready to talk about them. This willingness to express this indicates the development of insight and the sincere need to investigate the true source of these behaviours.
By stating that he will never behave like that again and that he will conform to society's norms, he is simply using expressions with no meaning to him. If he discusses seducing other adolescents, he is just manifesting the behaviour that led him to the hospital in the first place.