Restriction enzymes can also be used to generate compatible ends on PCR products. In all cases, one or more restriction enzymes are used to digest the DNA resulting in either non-directional or directional insertion into the compatible plasmid. The most common Type II enzymes are those like HhaI (NEB #R0139), HindIII (NEB #R0104), and NotI (NEB #R0189)
Answer: so in the beginning of mitosis there is only one nuclei but during telophase there are 2 nuclei
Explanation: I had the same exact question too but I did some research and I found the answer so here you go :) if I am missing anything please ask <3
B. The boiling point would be lower; water boils at lower temperatures at high altitudes.