Explanation:
this is the best I can do for you sorry let me know if this helps any.
When a stem cell divides, it first becomes an immature red blood cell, white blood cell, or platelet-producing cell. The immature cell then divides, matures further, and ultimately becomes a mature red blood cell, white blood cell, or platelet.
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
1. A forensic pathologist would most likely perform a forensic autospy. Forensic pathologists focus on cause of death by examining a bod or corpse. They perform postmortem examinations.
2. Insect activity will begin immediately if a body is left in the open. That is why insect activity can help investigations with determining certain things such as time of death, where the person died and the like.
3. A light meal will digest in about 1-2 hours. Digestion rates can give useful information about when a person had died. Medium meals take about 3-4 hours to digest and heavier meals take about 6 hours.
4. Indicative acts are actions or behaviors that may or may not have taken place before someone dies. Indicative is suggestive, so it may or may have happened. When you say indicative acts you are referring to what may have happened (or may not) based on certain circumstances, observations or evidence.
D surface tension because, adhesion is sticking, b makes no sense for this question, and heat has nothing to do with it either, because it distributes it's weight throughout its legs so it doesn't sink (it doesn't weigh much so this works.)
Hope this helps don't forget to hit that heart :)
Answer:
did you already get the answer or you still need help
