The scheme is shown below, the steps involved are as follow,
Step one: Reduction: The carbonyl group of given compound on reduction using
Wolf Kishner reagent converts the carbonyl group into -CH₂- group.
Step two: Epoxidation: The double bond present in starting compound when treated with
m-CPBA (<span>meta-Chloroperoxybenzoic acid) gives corrsponding epoxide.
Step three: Reduction: The epoxide is reduced to alcohol on treatment with
Lithium Aluminium Hydride (LiAlH</span>₄)<span> followed by hydrolysis.
Step four: Oxidation: The hydroxyl group (alcohol) is
oxidized to carbonyl (ketonic group) using oxidizing agent
Chromic acid (H</span>₂CrO₄).
The absorbance reported by the defective instrument was 0.3933.
Absorbance A = - log₁₀ T
Tm = transmittance measured by spectrophotometer
Tm = 0.44
Absorbance reported in this equipment = -log₁₀ (0.44) = 0.35654
True absorbance can be calculated by true transmittance, Tm = T+S(α-T)
S = fraction of stray light = 6%= 6/100 = 0.06
α= 1, ideal case
T = true transmittance of the sample
Tm = T+S(α-T)
now, T= Tm-S/ 1-S = 0.44-0.06/ 1-0.06 = 0.404233
therefore, actual reading measured is A = -log₁₀ T = -log₁₀ (0.404233)
i.e; 0.3933
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