Answer:
e. None of the above
Explanation:
For me as a Researcher, the reason could be increased Concentration of your DNA sample which you are using as your template. Try to decrease the concentration of DNA (up to 100 ng per reaction is enough and can increase up to 200 ng). so the reason for getting non specific bands is increase concentration of DNA which results in non specific amplification and also degradation of DNA in the reaction which you can see in your gel electrophoresis results.
i always corrected my results using the same technique that is lowering the concentration of DNA between 100 and 200 ng per single reaction of PCR.
Buddhism was the first of the great missionary faiths to take advantage of the mobility provided by the Silk Road to extend its reach far beyond its native ground. From its origins in north eastern India, Buddhism had already spread into the lands that are now Pakistan and Afghanistan by the 1st century BCE.
<span>The reserves will eventually be used up. </span>
D. The discoveries confirmed many but not all of the classifications that were based on observable traits, and scientists revised classifications when necessary.
