Part A
Plasmids are extracted from the bacteria
The plasmids are spliced at specific regions using restriction enzymes.
The cut plasmids are incubated with the novel gene and DNA ligase
Plasmids that will have incorporated the new gene are transformed back into the bacteria
Part B
The answer is transgenic organisms.
The hypothesis by Coleman was that the product of the ob+ gene was the appetite suppressing hormone. Hence, the homozygous ob/ob mutant are in a position to synthesize that hormone, and its circulating level would be zero. He also hypothesized that the product of the db+ gene was the receptor for the appetite-suppressing hormone. Thus, the homozygous db/db mutant would be able to synthesize the hormone but would not be in a position to respond to it. It would eat excessively and produce large amounts of body fat, which in turn would produce large amounts of appetite-suppressing hormone . In the absence of a receptor, the db/db mutant's hormone level would remain abnormally high.
Coleman's hypothesis were confirmed when the precise functions of the ob+ and db+ genes were determined. The peptide hormone encoded by the ob+ gene was named Leptin.
The transfer of genes from donor to recipient in microorganisms, where the recipient strain shows a change in genetic makeup at the end, is referred to as genetic recombination.
<h3>What is genetic recombination?</h3>
Genetic recombination is the formation of genetic combinations in offspring that are not present in the parents.
Recombination is a process by which pieces of DNA are broken and recombined to produce new combinations of alleles. This recombination process creates genetic diversity at the level of genes that reflects differences in the DNA sequences of different organisms.
In eukaryotic cells, which are cells with a nucleus and organelles, recombination typically occurs during the metaphase stage of meiosis.
The aim of genetic recombination is to produce offsprings with combinations of traits that differ from those found in either parent.
Learn more about recombination at: brainly.com/question/12685192
#SPJ1
Any chemical substance which forms naturally in the earth is called a mineral.
<h3>What is mineral?</h3>
A chemical compound having a specific crystal structure that occurs naturally in pure form or formed naturally in the earth is known as mineral.
So we can conclude that any chemical substance which forms naturally in the earth is called a mineral.
Learn more about mineral here: brainly.com/question/15844293
