The diaphragm is the primary muscle used in the process of inspiration, or inhalation
Cell-wall inhibiting antimicrobial drugs be less effective on gram-negative bacteria compared to gram-positive bacteria because the outer membrane of the gram-negative bacteria inhibits penetration of the drug and the peptidoglycan found in gram-positive bacteria is structurally different from that in gram-negative bacteria.
Answer: Option B & C
<u>Explanation:</u>
Antimicrobial drugs are induced into a body to act on that particular selective bacterium which causes disease. When antimicrobial drugs are injected they act efficiently on the gram positive bacteria inhibiting the proliferation of the cells by acting on the cell wall so that cell multiplication doesn’t happen.
On the other hand it is hard to act on the gram-negative bacteria as it has a cell membrane that inhibits drug penetration into it. Both cell walls contain peptidoglycan but in the gram-positive is more assembled and layered while in the gram-negative it is just a thin layer. As gram-positive is thick layered it provides place for another molecule to attach to it but the thin layer in gram-negative inhibits it.
Griffith's experiment worked with two types of pneumococcal bacteria (a rough type and a smooth type) and identified that a "transforming principle" could transform them from one type to another.
At first, bacteriologists suspected the transforming factor was a protein. The "transforming principle" could be precipitated with alcohol, which showed that it was not a carbohydrate. But Avery and McCarty observed that proteases (enzymes that degrade proteins) did not destroy the transforming principle. Neither did lipases (enzymes that digest lipids). Later they found that the transforming substance was made of nucleic acids but ribonuclease (which digests RNA) did not inactivate the substance. By this method, they were able to obtain small amounts of highly purified transforming principle, which they could then analyze through other tests to determine its identity, which corresponded to DNA.