Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
Answer:
Both no
Explanation:
be because it is liquid, they don't have fix shape
When a small populations' gene frequencies are changed drastically by chance alone it is called a genetic drift
Answer: C. A brood chamber is made by bees, but comb foundations are built by humans.
Explanation:
The brood chamber is a natural part of the beehive where the bee workers made cells where these works lay eggs. Here, development of the larvae and pupae takes place. The cells of the brood chamber holds the pollen, nectar and honey which is used to feed to develop the larvae.
Comb foundations are the artificial creation made by humans is a place made from wax which forms the base of the honeycomb. It is used to give the support to the beehive.
