Hi,
I think the answer you're looking for is already in the question you submitted. However, I disagree that a frameshift could result from the mutation because frameshifts would only happen if there were insertions or deletions in the sequence. For your question, this is an example of a simple point mutation/nucleotide base substitution, and would only result to either a missense or a nonsense mutation. Depending on the bases flanking your mutation, this results to transcribing a different sequence of RNA leading to translating either a different amino acid or a stop codon.
Answer:
Translation uses DNA as a template to direct the synthesis of a RNA which in turn serves as a template for Translation which template is used to direct the synthesis of a polypeptide chain.
Without transcription, there will not be a mRNA strand to act as a template for translation by the ribosomes to occur at all.
The right answer is C) vector
In molecular biology and genetic engineering, vectors are DNA molecules that allow the propagation of sequences of interest. These are chimeric DNA molecules such as plasmids or artificial bacterial chromosomes, containing an origin of replication and one or more genetic markers. The origin of replication allows the maintenance of the vector in the target cell during generations.