The Michaelis–Menten equation is an expression of the relationship between the initial velocity ????0 of an enzymatic reaction a
nd substrate concentration [S] . There are three conditions that are useful for simplifying the Michaelis–Menten equation to an expression from which the effect of [S] on the rate can be more readily determined. Match the condition (e.g., [S]=Km ) with the statement or statements that describe it. (1) Doubling [S] will almost double the rate.
(2) Half of the active sites are occupied by substrate.
(3) About 90% of the active sites are occupied by substrate.
(4) Doubling [S] will have little effect on the rate.
(5) Less than 10% of the active sites are occupied by substrate.
(6) This condition will result in the highest rate.