Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR
The Answer IS AEROBIC RESPIRARTION because it’s The breakdown of food to create energy in the presence of oxygen.
There are three types of energy involved in a muscle's contraction. There is metabolism, catabolism, and anabolism. Metabolism is the events that are carried out in the human body to create energy and other things needed for activity. Catabolism is the process during the organic matter is broken down and the energy is released, it takes place during increased movement. Anabolism is the energy-consuming process that substances are created, it takes place when there is little movement. All three of these work to gather in muscle contraction.
False Routine maintenance costs on a hybrid may be lower than on a regular car.
Answer:
Microblog
Explanation:
Microblog can be describes as a mixture of both blogging as well as instant messaging. Microblogging allows the users to share short messages as well as it also allows posting of these messages with an online audience. The content of micro blogging is much smaller as compared to the traditional blogging . The actual as well as the aggregated sizes of files of microblogging are much shorter than the tradiotional bloggings.
