They use the directions found in DNA to make Proteins & Polypeptides.
The expected result of this poison on secondary active transport is that the poison will prevents the conformational changes which are necessary for the pump to perform its function, thus preventing it from been able to carry out transportation of ions in and out of the cell.
Answer:
DNA is read in the 3 prime to 5 prime direction, and RNA is read 5 to 3.