For obtaining an adequate amount of DNA samples for DNA fingerprinting, polymerase chain reaction (PCR) can be used.
Polymerase chain reaction (PCR) is a technique that is used to amplify the DNA sample. The steps included in PCR are:-
1. Denaturation of the double-stranded DNA (at around 95°C)
2. Annealing of the primers to each of the single-stranded DNA templates ( at around 65°C)
3. Extension or elongation of the primers by considering every single-stranded DNA as a template. This elongation is carried out by <em>Taq </em>polymerase (at around 72°C).
With each cycle of PCR, the DNA sample is increased by 2ⁿ. Here, 'n' represents the number of PCR cycles.
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