Answer:
Since high ethanol is a major stress during ethanol fermentation, ethanol-tolerant yeast strains are highly desirable for ethanol production on an industrial scale. A technology called global transcriptional machinery engineering (gTME), which exploits a mutant SPT15 library that encodes the TATA-binding protein of Saccharomyces cerevisiae (Alper et al., 2006; Science 314: 1565-1568), appears to be a powerful tool. to create ethanol tolerant strains. However, the ability of the strains created to tolerate high ethanol content in rich media remains to be demonstrated. In this study, a similar strategy was used to obtain five strains with higher ethanol tolerance (ETS1-5) of S. cerevisiae. When comparing the global transcriptional profiles of two selected strains ETS2 and ETS3 with that of the control, 42 genes that were commonly regulated with a double change were identified. Of the 34 deletion mutants available in an inactivated gene library, 18 were sensitive to ethanol, suggesting that these genes were closely associated with tolerance to ethanol.
Explanation:
Eight of them were novel and most were functionally unknown. To establish a basis for future industrial applications, the iETS2 and iETS3 strains were created by integrating the SPT15 mutant alleles of ETS2 and ETS3 into the chromosomes, which also exhibited increased tolerance to ethanol and survival after ethanol shock in a rich medium. Fermentation with 20% glucose for 24 h in a bioreactor revealed that iETS2 and iETS3 grew better and produced approximately 25% more ethanol than a control strain. The performance and productivity of ethanol also improved substantially: 0.31 g / g and 2.6 g / L / h, respectively, for the control and 0.39 g / g and 3.2 g / L / h, respectively, for iETS2 and iETS3.
Therefore, our study demonstrates the utility of gTME in generating strains with increased tolerance to ethanol that resulted in increased ethanol production. Strains with increased tolerance to other stresses such as heat, fermentation inhibitors, osmotic pressure, etc., can be further created using gTME.
Answer:
1. water, CO2 and Light energy
2. the runner's cells are making up for an oxygen deficit
3. chloroplasts absorb sunlight
4. carbon dioxide
5. eukaryotes
Explanation:
Answer:
okay so im guessing they want you to compare 2 different land resources. Say a water power station and a coal mine for example.
you could state the different effects the coal mine would have in comparison to the water station.
the coal mine say has pollution produced as a result in producing the energy. Whereas water has a lot better environmental value, but might cost more to produce energy
there is a lot of information of the 2 on google :)
Surface area is an important factor in limiting cell growth because
a. the cell can burst if the membrane becomes too large.
b. materials cannot enter the cell if the surface is too large.
c. the cell may become too large to take in enough food and to remove enough wastes.
d. waste products cannot leave the cell if the cell is too small.
Answer:
c. the cell may become too large to take in enough food and to remove enough wastes.
Explanation:
The exchange of substances with surroundings is done by cells by the process of diffusion. The rate of diffusion of substances requires the presence of a higher surface area than volume. As the cells become larger, their surface area is reduced with respect to the volume. Therefore, larger cells can not support the diffusion of substances in and out as required for its proper functioning. If cells are allowed to grow continuously, they will not be able to intake the requires materials such as food and to release the waste outside.
Answer:
The first genetic code is used to determine the nucleotide triplets that code for amino acids.
Explanation:
The second genetic code is used by the amino acid synthetase in order to determine whether the correct tRNA is being used for it's corresponding amino acid.
