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<span>Marine ecosystems like lakes and oceans have aphotic zones. Aphotic zones refer to the zones in the water where there is little or no sunlight. It is found in bodies of water were depths only receive less than 1% of sunlight penetrations. Bioluminescence is essentially the only light found in this zone and most food comes from dead organisms that sink at the bottom of lakes or oceans. </span>
Answer:
Short answer is primers are partially complementary.
Explanation:
Forward primer: 5'-AGTCTACTCGTAACCGGTTACC-3'
Reverse primer: 5'-TAAGGCATCATGGTAACCGGTT-3'
When we write reverse primer 5' to 3' we can easily see that
3'-TTGGCCAATGG---5' is complementary to the forward primers'
5'---AACCGGTTACC-3' sequence. So instead of binding to the template DNA these primers might bind each other resulting with reduction of efficiency of DNA amplification.