In the presence of oxygen, the three-carbon compound pyruvate can be catabolized in the citric acid cycle. First, however, the p
yruvate (1) loses a carbon, which is given off as a molecule of CO2, (2) is oxidized to form a two-carbon compound called acetate, and (3) is bonded to coenzyme A.
Explanation: This reaction is catalyzes by pyruvate dehydrogenase. Pyruvate being the end product of glycolysis has many fates after glycolysis,one of which is to enter the TCA(Tricarboxylic acid cycle) cycle. It is first converted to actetate by the action of pyruvate dehydrogenase. This enzyme converts pyruvate to acetate releasing CO2 and NADH because this oxidative decarboxylation of pyruvate is coupled with reduction of NAD+ which can feed into the electron transport chain.
Student 2 repeated the experiment several times with different seeds to make sure the experiment would come out with the same answers and was reliable, using the same area would make sure the environment wouldn't interfere. The other students didn't do all the things that student 2 needed for the experiment.