Answer:
Given:
Number of colonies on plate = 40
Amount of DNA used for transformation = 100ng
Volume of competent cells used = 100ul
Total volume = 1ml (1000ul)
Volume plated for growth = 200ul
The amount of cells transformed per 1 µg of DNA is called the transformation efficiency
(CFU is colony forming units) => (number of colonies on plate/ng of DNA plated) X 1000 ng/µg = CFU/µg of DNA
Amount of DNA used for plating = 100ng * (200/1000)
= 100ng * (1/5)
= 100/5 = 20ng
Efficiency calculation = 40 colonies / 20ng = 2 colonies/ng
(2 colonies/ng) * (1000 ng/µg) = 2000 CFU/ug DNA
Transformation efficiency = 2000 Transformants / ug DNA
= 2 x 10³ Transformants / ug DNA
Explanation:
Efficiency of transformation is highest in the 100 pg-1 ng range, so Low transformation efficiency could be because of high amount of DNA used for transformation.
Science can be applied to many things the earth around us. Maybe even technology for example self-driving cars is a part of science!
Answer:
C (Chlorophyll)
Explanation:
Adenosine triphosphate (ATP) is an energy carrying molecule in all living cells. It is the usable form of energy by cells. Organisms are able to carry out cellular functions using the energy carried by ATP molecules. ATP like other molecules is made up of components that make up its chemical structure.
ATP is composed of three structural components; A five-carbon sugar called RIBOSE at the core of its structure, attached to a nitrogenous base called Adenine and phosphate group (three in ATP). The energy in ATP is released when it loses its phosphate component.
Chlorophyll is a pigment found in the Chloroplast of plant cells. It is another molecule that is made up of its own components e.g nitrogen, magnesium etc.
Wind because its not mechanically eroding them..