Glycolysis will produce 2 NADH (reducing agents) from the 6th reaction involving the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate by the enzyme glyceraldehyde-3-phosphate dehydrogenase. 2 ATP and 2 pyruvate is yielded by the last reaction of glycolysis which is the conversion of phosphoenolpyruvate to pyruvate by the enzyme pyruvate kinase.
