Answer:
<u>During Metaphase</u>
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Explanation:
During Metaphase the cell chromosome align themselves in the middle of the cell.This occur due to a cellular process called "Tug of War".
The chromosome which have been replicated and joined at the central point called centromere are called sister chromatids
Prior to metaphase , Kinetochore type of protein are formed around the centromere. Long protein filament called kinetochore are extended from poles to other end of the cell attached to kinetochore.
Therse is important checkpoint in the middle of mitosis called<u> metaphase.</u>
At this point cell ensure that , it is ready to divide or not.
Once the cell ensure that everything is ready to divide. Only after then , yhe cell enters the fourth phase called <u>anaphase.</u>
0.1 mL of the stock solution of the enzyme is taken and made up to 5.0 mL with 0.001M HCl in order to prepare a 50-fold diluted enzyme.
<h3>What is dilution?</h3>
Dilution is a process of making a solution of lower concentration from a solution of higher concentration by the addition of solvent to a given volume of the solution of higher concentration.
Dilution of solutions is done using the dilution formula in order to determine the given volume of diluent or stock solution required. The dilution formula is given below:
where:
- C1 = Initial concentration of enzyme
- C2 = Final concentration of enzyme
- V1 = Initial volume
- V2 = Final volume
For the enzyme dilution;
C1 = 1 mg/mL
C2 = 1/50 mg/ml = 0.02 mg/ml
V= ?
V2 = 5 ml
V1 = C2V2/C1
V1 = 0.02 * 5/1 = 0.1 mL
Therefore, 0.1 mL of the stock solution of the enzyme is taken and made up to 5.0 mL with 0.001M HCl in order to prepare a 50-fold diluted enzyme.
Learn more about dilution at: brainly.com/question/24881505
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Lipids serve as structural components of cell membranes, function as energy storehouses, and function as important signalling molecules. They include fats, waxes, oils, hormones, and certain components of membranes and function as energy-storage molecules and chemical messengers.
Hope this helped :)
Answer:
D. a nonpolar covalent bond
