The US supreme court establish the precedent allowing civilians to bring a suit against federal law enforcement officers and other government employees under 42 US section C 1983 during the case of WEBSTER BIVENS VS 6 UNNAMED FBI AGENTS and suit against federal law enforcement.
<h3>What is the law under 42 US section C of 1983?</h3>
Section C of 1983 gives individuals the right to sue the federal and state government employees and law enforcement officers acting under color of state law for violations of their civil rights.
For this section, its purpose was derived from the case between Webster Bivens and 6 unnamed FBI agents after a complaint that these agents made a warrantless entry to search his apartment.
Learn more on US federal laws here: brainly.com/question/25912245
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Your answer is going to be C I have a 100% in biology
The answer is prophase of meiosis I.
Meiosis is a reducing cell division during which the number of chromosomes is reduced by half in the daughter cells. It consists of meiosis I and meiosis II. Major gene reshuffling takes place during meiosis I. This exchange of genetic material between homologous chromosomes is known as the crossover. Homologous chromosomes are present only in the meiosis I. They pair up during prophase of meiosis I. When they are paired up during prophase I, it is obviously that gene reshuffling takes place during prophase I.
Answer/Explanation:
(1) a mutation in the coding region, resulting in an inactive protein
To check to see if there is a mutation, you could extract the DNA from the cancer cells and then perform PCR to amplify the gene of interest. You could then perform sanger sequencing and compare the sequence to the normal gene to see if a mutation is present. To test the effect of the mutation, you would want to see if an active protein has been formed.
To see if a normal sized protein has been formed, you could perform a western blot, comparing the protein band to the WT protein band. If the protein is absent or much smaller, it is likely not a functional protein.
(2) epigenetic silencing at the promoter of the gene, resulting in reduced transcription.
To check for changes in the epigenetic landscape of the promoter, you could perform chromatin immunoprecipitation by extracting the chromatin from the tumour cells and using antibodies for different chromatin marks to see what has changed between the normal cells and the tumor cells. E.g. H3K9me3, H3K27me3. You would perform a pull down with the antibody of interest and then PCR for your promoter to specifically look at changes at that gene compared to normal cells. To test DNA methylation, you could perform bisulfite sequencing.
To see how transcription is affected, you could extract RNA from the tumor and normal cells, and compare the levels of RNA between the two samples by qRT-PCR