The best and most correct answer among the choices provided by the question is <span>How did the hole in the ozone layer form?</span> Hope my answer would be a great help for you. If you have more questions feel free to ask here at Brainly.
The science can only answer the things that has some scientific reasons behind that. It cannot answer the hypothetical questions and the subject not related to science.
The question that when is creating a new vaccine appropriate? How did the hole in the ozone layer form?
This question has some facts behind it that can be answered by the help of science. We can use scientific methods to inquire about the answer.
Cation-exchange chromatography is used when the molecule of interest is positively charged, the stationary phase is negatively charged and positively charged molecules are loaded to be attracted to it. So, the amino acids with negative charge will elute the first. Glutamate, leucin, arginine is the order of elution because of their pI values ~3, ~6 ~10.
