After being divided into two halves, the sample from lane two may still be present in lane 4, which is possible. Lane 2 is around 700 base pairs long, and lane 4 will add up to about 700 base pairs as well, despite the restriction enzyme that divides the DNA into two parts.
What is gel electrophoresis?
- A laboratory technique called gel electrophoresis is used to divide combinations of DNA, RNA, or proteins based on their molecular sizes.
- In gel electrophoresis, the molecules that need to be separated are forced through a gel that has tiny holes by an electrical field.
- It involves an electrical field, specifically one that is applied such that the gel has a positive charge on one end and a negative charge on the other.
- Negatively charged molecules like DNA and RNA will be drawn to the positively charged end of the gel.
- However, because proteins lack a negative charge, they must first be combined with sodium dodecyl sulfate, a detergent, in order to separate them using this method.
- The proteins are given a linear structure and a negative charge during this process, enabling them to move toward the gel's positive end and be separated.
- Finally, after utilizing it to separate the DNA, RNA, or protein molecules, bands representing of different molecular size
Principle of gel electrophoresis:
Since DNA is negatively charged, it will move toward the positively charged electrode when an electric current is supplied to the gel. The DNA fragments are organized in size order because shorter DNA strands travel through the gel more quickly than longer ones.
Steps in gel electrophoresis:
- Pouring the gel first,
- getting your samples ready,
- the gel must be loaded,
- run, stained,
- exposed to an electric field while doing so.
Hence all about the gel electrophoresis.
To learn more about gel electrophoresis click on the link
https://brainly.in/question/6879234
#SPJ4
