When growing cultures, the agar is<u> inoculated</u> to separate pathogenic colonies of organisms from colonies of normal flora.
Agar is the medium that is used for growing natural, common flora of microorganisms as the majority of the microorganisms can sustain in the agar medium.
If we want to determine a separate pathogenic colony of organisms in an agar medium then we need to inoculate the agar growth medium so that the pathogenic organism is separated from the normal flora of the microorganism. This technique allows us to filter out the cells that have the pathogenic microorganism in it.