Helicases move along one strand of the duplex and push the opposing strand aside. They either migrate in the 3'-5' or 5'-3' orientation along the DNA or RNA (some can move in either direction).
Living things need helicases, which are enzymes, to separate the double-stranded DNA in this instance from other nucleic acid strands.
Helicases accomplish this by severing the hydrogen bonds that link the two strands.
The DNA double helix's two strands are initially separated by an enzyme known as a DNA helicase.
Each DnaB helicase winds the parental duplex by encircling one strand of the duplex DNA and moving in the 5′ to 3′ direction with respect to this ssDNA.
Primers are created on occasion when DnaB interacts with primase.
