The correct sequence of these events (origin of mitochondria, origin of multicellular eukaryotes, origin of chloroplasts, origin of cyanobacteria, origin of fungal-plant symbioses), from earliest to most recent, in the evolution of life on earth is 4, 3, 1, 2, 5.
The transition to eukaryotic cells seems to have taken place in the Proterozoic Era, around 1.2 to 1.5 billion years ago. Nevertheless, the latest genetic studies propose the divergence of eukaryotes from prokaryotes nearer to 2 billion years ago.
Margulis suggested the mechanism of endosymbiosis, to describe the origin of mitochondria as well as chloroplasts from persistent living prokaryotes. In accordance with this idea, a bigger prokaryote engulfed or enveloped a smaller prokaryote around 1.5 billion to 700 million years ago.
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